| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carsillo, Ava C (8)
|
ADDELM |
36.36 |
36.39 |
0.03 |
| 2 |
Laurie, Josephine S (8)
|
ADHAWK |
47.14 |
40.97 |
-6.17 |
| 3 |
Fraley, Ella R (8)
|
ADBFIN |
45.16 |
41.14 |
-4.02 |
| 5 |
Downey, Isabelle G (8)
|
ADSARY |
45.76 |
42.45 |
-3.31 |
| 6 |
Zhang, Annabelle Y (7)
|
ADCPP |
50.45 |
42.49 |
-7.96 |
| 7 |
Pickering, Michelle E (8)
|
ADBSPA |
48.77 |
44.11 |
-4.66 |
| 8 |
Stoup, Madelyn L (8)
|
ADCPP |
41.93 |
45.27 |
3.34 |
| 9 |
Ruscio, Emily A (7)
|
ADSCHE |
|
45.32 |
- |
| 10 |
Huang, Rylie Z (8)
|
ADSSCY |
46.62 |
45.97 |
-0.65 |
| 11 |
Cohen, Evyn B (8)
|
ADCLIN |
47.10 |
46.90 |
-0.20 |
| 12 |
Bradley, Jade C (7)
|
ADSCHE |
|
47.45 |
- |
| 13 |
Deal, Grace A (8)
|
ADCLIN |
46.60 |
47.76 |
1.16 |
| 14 |
Adams, Michelle E (6)
|
ADCPP |
|
48.42 |
- |
| 15 |
Braun, Julia L (7)
|
ADNTS |
1:01.65 |
48.57 |
-13.08 |
| 16 |
Eldred, Carly J (8)
|
ADGLFY |
50.17 |
50.20 |
0.03 |
| 17 |
Hovak, Madelyn E (8)
|
ADNHA |
|
50.68 |
- |
| 18 |
Schwarz-Eise, Celeste F (8)
|
ADUSAM |
57.45 |
50.74 |
-6.71 |
| 19 |
Ryan, Chloe M (7)
|
ADUSAM |
|
55.16 |
- |
| 20 |
Ryan, Callie J (7)
|
ADUSAM |
|
57.62 |
- |
| 21 |
Shukla, Aarya O (7)
|
ADSCHE |
|
1:00.33 |
- |
| 22 |
Brandl, Kristin W (8)
|
ADBSPA |
|
1:00.40 |
- |
| 23 |
MacLeod, Addison M (6)
|
ADUSAM |
1:07.00 |
1:02.56 |
-4.44 |
| 24 |
Marra, Gia V (6)
|
ADUSAM |
|
1:03.49 |
- |
| 25 |
Sekelj, Ella S (6)
|
ADNTS |
|
1:07.23 |
- |
| 26 |
O'Donnell, Bridgett M (7)
|
ADEELS |
1:21.30 |
1:09.31 |
-11.99 |
| 27 |
Arnold, Avery S (8)
|
ADUSAM |
|
1:19.20 |
- |
|
Chen, You-Lin (8)
|
ADSARY |
48.56 |
NS |
- |
|
D'Amato, Victoria (8)
|
ADALBS |
52.30 |
NS |
- |